Selected Publications

2019

Katiyar, A., Tocco, V.J., Li, Y., Aggarwal, V., Tamashunas, A., Dickinson, R.B., Lele, T.P., 2019, Nuclear size changes caused by local motion of cell boundaries unfold the nuclear lamina and dilate chromatin and intranuclear bodies, Soft Matter, Advance Article.  PDF

Lele, T.P., Brock, A., Peyton, S., 2019, Emerging concepts and tools in cell mechanomemory, Annals of Biomedical Engineering.(accepted).

Chawla, R., Gupta, R.P., Lele, T.P., Lele, P., 2019, A skeptic’s guide to bacterial mechanosensing, Journal of Molecular Biology.(accepted).

Zhang, Q., Narayanan, V., Mui, K.L., O’Bryan, C.S., Anderson, R.H., Birendra, K.C., Davis, J.I., Denis, K.B., Antoku, S., Roux, K.J., Dickinson, R.B., Angelini, T.E., Gundersen, G.G, Conway, D.E., Lele, T.P., 2019, Mechanical stabilization of the glandular acinus by linker of nucleoskeleton and cytoskeleton complex, Current Biology, 29(17):2826-2839.  PDF

Bennett, R., Bele, A., Small, E., Will, C., Nabet, B., Oyer, J., Huang, X., Ghosh, R., Grzybowski, A., Yu, T., Zhang, Q., Riva, A., Lele, T.P., Schatz, G., Kelleher, N., Ruthenburg, A., Liphardt, J., Licht, J., 2019, A Mutation in Histone H2B Represents a New Class of Oncogenic Driver, Cancer Discovery, 9(10):1438-1451.  PDF

Agrawal, A., Lele, T.P., 2019, Mechanics of the nuclear membranes, Journal of Cell Science, 132(14).  PDF

Halfmann, C., Sears, R., Katiyar, A., Busselman, B., Aman, L., Zhang, Q., O’Bryan, C., Angelini, T.E., Lele, T.P., Roux, K., 2019, Repair of nuclear ruptures requires barrier-to-autointegration factor, Journal of Cell Biology, 218(7):2136-2149.  PDF

Kent, I., Zhang, Q., Katiyar, A., Li, Y., Pathak, S., Dickinson, R.B., Lele, T.P., 2019, Apical cell protrusions cause vertical deformation of the soft cancer nucleus, Journal of Cellular Physiology, 234(11):20675-20684.  PDF

2018

Zhang, Q., Tamashunas, A., Agrawal, A., Torbati, M., Katiyar, A., Dickinson, R.B., Lammerding, J., Lele, T.P., 2018, Local, transient tensile stress on the nuclear membrane causes membrane rupture, Molecular Biology of the Cell, 30(7):899-906.  PDF

Lele, T.P., Dickinson, R.B., and Gundersen, G.G., 2018, Mechanical principles of nuclear shaping and positioning, Journal of Cell Biology, 217(10):3330-3342.  PDF

Chukkapalli, S., and Lele,T.P. 2018, Periodontal cell mechanotransduction, Open Biology., 8(9):180053.  PDF

Zhang, Q., Tamashunas, A., and Lele, T.P. (2018), A Direct Force Probe for measuring mechanical integration between the nucleus and cytoskeleton, Journal of Visualized Experiments, (137):58083.

Book Chapter
Tocco V.J., Neelam S., Zhang Q., Dickinson R.B., Lele T.P. (2018) Direct Force Probe for Nuclear Mechanics. In: Gundersen G., Worman H. (eds) The LINC Complex, vol 1840, Methods in Molecular Biology, Humana Press, New York, pp. 81-90.

2017

Tocco, V.J., Li, Y., Christopher, K.G., Matthews, J.H., Aggarwal, V., Paschall, L, Luesch, H., Licht, J.D., Dickinson, R.B., and Lele, T.P. 2017, The nucleus is irreversibly shaped by motion of cell boundaries in cancer and non-cancer cells, Journal of Cellular Physiology. 233(2):1446-1454. JCP spotlight article    PDF

Aggarwal, V., and Lele, T.P. 2017, Intracellular concentration gradients that mirror external gradients in microfluidic flows: a computational analysis, Cellular and Molecular Bioengineering, 10(2):1-10.   PDF

Kent, I. and Lele, T.P. 2017. Microtubule-Based Force Generation, WIREs Nanomed Nanobiotechnol, 9(3).   PDF

2016

Alam, S., Zhang, Q., Prasad, N., Li, Y., Chamala, S., Kuchibhotla, R., KC, B., Aggarwal, V., Shrestha, S., Jones, A., Levy, S., Roux, K., Nickerson, J., and Lele, T.P., 2016, The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity, Scientific Reports, 6:38063.    PDF

Torbati, M., Lele, T.P., and Agrawal, A., 2016, On the Ultra-Donut Topology of The Nuclear Envelope, Proceedings of the National Academy of Sciences, 113(40):11094-11099.    PDF

Kent, I., Rane, P.S., Dickinson, R.B., Ladd, A.J.C., and Lele, T.P., 2016, Transient Pinning and Pulling: A Mechanism for Bending Microtubules, 11(3):e0151322.

Aggarwal, V., Dickinson, R.B., and Lele, T.P., 2016, Concentration sensing by the moving nucleus in cell fate determination: a computational analysis, 11(2): e0149213.    PDF

Torbati, M., Lele, T.P., and Agrawal, A., 2016, An unresolved LINC in the nuclear envelope. Cellular and Molecular Bioengineering, 9(2):252–257.    PDF

Neelam, S., Hayes, P., Zhang, Q., Dickinson, R. B., and Lele, T. P., 2016, Vertical uniformity of cells and nuclei in epithelial monolayers, Scientific Reports, 6:19689.   PDF  F1000Prime

Zhang, Q., Kota, K. P., Alam, S. G., Nickerson, J. A., Dickinson, R. B., and Lele, T. P., 2016, Coordinated dynamics of RNA splicing speckles in the nucleus. Journal of Cellular Physiology, 231: 1269–1275.  Article Highlighted and chosen for Cover Art.    PDF

Neelam, S., Dickinson, R.B., and Lele, T.P., 2016, New approaches for understanding the nuclear force balance in living, adherent cells, Methods 94 (2016) 27–32.    PDF

2015

Dickinson, R.B., Neelam, S., and Lele, T.P., 2015, Dynamic, mechanical integration between nucleus and cell- where physics meets biology, Nucleus, 6(5):360-365.    PDF

Li, Y., Lovett, D., Zhang, Q., Neelam, S., Kuchibhotla, R.A., Zhu, R., Gundersen, G.G., Lele, T.P., and Dickinson, R.B., 2015, Moving cell boundary drives nuclear flattening during cell spreading, Biophysical Journal, 109(4):670-686.    PDF

Dickinson, R.B., and Lele, T.P., 2015, Chemical engineering principles in the field of cell mechanics, Industrial & Engineering Chemistry Research, 54(23):6061–6066.

Neelam, S., Chancellor, T.J., Li, Y., Nickerson, J., Roux, K., Dickinson, R.B., and Lele, T.P., 2015, A direct force probe reveals the mechanics of nuclear homeostasis in the mammalian cell, Proceedings of the National Academy of Sciences, 112(18):5720-5725.    PDF  F1000Prime

Alam, S.G., Lovett, D., Kim, D.I., Roux, K., Dickinson R.B., and Lele T.P., 2015, The nucleus is an intracellular propagator of tensile forces in NIH 3T3 fibroblasts, Journal of Cell Science, 128(10):1901-1911. Highlighted by the journal in its ‘In this issue’ section.  PDF

2014

Wu J., Kent I.A., Shekhar N., Chancellor T.J., Mendonca A., Dickinson R.B., and Lele T.P., 2014, Actomyosin pulls to advance the nucleus in a migrating tissue cell, Biophysical Journal, 106:7-15.    PDF

Book Chapter
Alam S. G., Lovett D., Dickinson R.B., Roux K., and Lele T.P., Nuclear Forces and Cell Mechanosensing. In: Engler A.J and Kumar S., eds.: Mechanotransduction

2013

Lewis J.S., Dolgova N., Chancellor T.J., Acharya A.P., Karpiak J.V., Lele T.P., Keselowsky B.G., 2013, Dendritic cell activation is influenced by cyclic mechanical strain when cultured on adhesive substrates, Biomaterials, 34(36):9063–9070.    PDF

Shekhar N., Neelam S., Wu J., Ladd A., Dickinson R.B., and Lele T.P., 2013, Fluctuating Motor Forces Bend Growing Microtubules, Cellular and Molecular Bioengineering, 6(2):120-129. One of four award publications chosen by CMBE for 2013.    PDF

Lovett D.B., Shekhar N., Nickerson J.A., Roux K., Lele T.P., 2013, Modulation of nuclear shape by substrate rigidity, Cellular and Molecular Bioengineering, 6(2):230-238.    PDF  F1000Prime

Shekhar N., Wu J., Dickinson R.B. and Lele T.P., 2013, Cytoplasmic Dynein: Tension Generation on Microtubules and the nucleus, Cellular and molecular Bioengineering, 6(1):74-81.    PDF

2012

Wu J., Shekhar N., Lele P.P., Lele T.P., 2012, FRAP Analysis: Accounting for Bleaching during Image Capture, 7(8):e42854.    PDF

Wu J. Dickinson R.B., Lele T.P., 2012, Investigation of in vivo Microtubule and Stress Fiber Mechanics with Laser Ablation, Integrative Biology, 4:471–479.    PDF

2011

Wu J., Misra G., Russell R. J., Ladd A., Lele T.P., Dickinson R.B., 2011, Effects of Dynein on Microtubule Mechanics and Centrosome Positioning. Molecular Biology of the Cell, 22(24):4834-4841.    PDF

Wu J., Lee K.C., Dickinson R.B., Lele T.P., 2011, How Dynein and Microtubules Rotate the Nucleus, Journal of Cellular Physiology, 226:2666-2674.  Highlighted by the journal.    PDF

Russell R.J., Grubbs A., Mangroo S., Nakasone S., Dickinson R.B. and Lele T.P., 2011, Sarcomere Length Fluctuations and Flow in Capillary Endothelial Cells, Cytoskeleton, 68(3):150-6.    PDF

2010

Lee J., Wang Y., Ren F. and Lele T.P., 2010, A Stamp Wound Assay for Studying Coupled Cell Migration and Cell Debris Clearance, Langmuir, 26(22):16672-6.    PDF

Lee J, Chu B.H., Sen S., Gupte A., Chancellor T.J., Chang C.-Y., Ren F., Kumar S., and Lele T.P., 2010, Modulating malignant epithelial tumor cell adhesion, migration, and mechanics with nanorod surfaces, Biomedical Microdevices, 13(1):89-95.    PDF

Chancellor T.J., Lee J., Thodeti C. and Lele T.P., 2010, Actomyosin tension exerted on the nucleus through nesprin-1 connections influences endothelial cell adhesion, migration and cyclic strain induced reorientation, Biophysical Journal, 2010 Jul 7;99(1):115-23.    PDF  F1000Prime

Zaveri T.D., Dolgova N.V., Chu B.H., Lee J., Wong J., Lele T.P., Ren F., Keselowsky B.G., 2010, Contributions of surface topography and cytotoxicity to the macrophage response to zinc oxide nanorods, Biomaterials, 31(11):2999-3007.

2009

Russell R.J., Xia S-L, Dickinson R.B. and Lele T.P., 2009, Sarcomere mechanics in capillary endothelial cells, Biophysical Journal, 97(6):1578-1585.    PDF

Lee J., Chu B.H., Chen K.H, Ren F., Lele T.P., 2009, Randomly oriented, upright SiO2 coated nanorods for reduced adhesion of mammalian cells, Biomaterials, 30:4488–4493.    PDF

2008

Lee J., Kang B.S., Chancellor T.J., Chu B.H., Wang H.T., Keselowsky B., Ren F. and Lele T.P., 2008, The control of cell adhesion and viability by zinc oxide nanorods, Biomaterials, 29:3743–3749.    PDF

Luo Y., Xu X., Lele T.P., Kumar S. and Ingber D.E., 2008, A multimodular tensegrity model for an actin stress fiber, Journal of Biomechanics, 41(11):2379-87.     PDF

Lele TP, Thodeti C, Pendse J and Ingber DE, Investigating complexity of protein-protein interactions in focal adhesions, Biophysical and Biochemical Research Communications, 369(3):929-34.    PDF

2007

Kang BS,Wang HT, Lele TP, Ren F, Pearton SJ, Johnson JW, Rajagopal P, Roberts JC, Piner EL and Linthicum KJ, 2007, Prostate specific antigen detection using AlGaN/GaN high electron mobility transistors, Applied Physics Letters, 91(11).    PDF

Pearton S, Lele TP, Tseng Y and Ren F, 2007, Penetrating living cells with semiconductor nanowires, Trends in Biotechnology, 25(11):481-2.    PDF

Lele TP, Sero JE, Matthews BD, Kumar S, Xia S, Montoya-Zavala M, Polte T, Overby D, Wang N, Ingber DE, 2007, Tools to study cell mechanics and mechanotransduction, Methods in Cell Biology, 83:441-72.    PDF

Lele TP and Kumar SK, 2007, Brushes, cables and ratchets: Recent insights into multiscale assembly and mechanics of cellular structural networks, Cell Biochemistry and Biophysics, 47: 348-360.    PDF

Chancellor TJ, Russell R and Lele TP, 2007, Toward in-situ biochemistry: combining chemical kinetics approaches with bio-molecular imaging in living cells, Biotechnology Progress, 24(1):89-95.    PDF

Wang HT, Kang BS, Chancellor TJ, Lele TP, Tseng Y, Ren F, Pearton SJ, Johnson JW, Rajagopal P, Roberts JC, Piner EL and Linthicum KJ, 2007, Selective Detection of Hg(II) ions from Cu(II) and Pb(II) Using AlGaN/GaN High Electron Mobility Transistors, Electrochemical and Solid-State Letters,     PDF

Pearton SJ, Ren F, Kang BS, Wang HT, Gila BP, Norton DP, Tien LC, Chancellor TJ, Lele TP, Tseng Y and Lin J, GaN and ZnO based sensors forBio, Nuclear Materials and Chemical Detection, Proceedings of the Electrochemical Society Meeting, Washington, 2007

Wang HT, Kang BS, Chancellor TJ, Lele TP, Tseng Y, Ren F, Pearton SJ, Johnson JW, Rajagopal P, Roberts JC, Piner EL and Linthicum KJ, 2007, Fast electrical detection of Hg(II) ions with AlGaN/GaN high electron mobility transistors, Applied Physics Letters, 91(4).    PDF

Book Chapter
Goldman W, Bechinger B and Lele TP, Cytoskeletal proteins at the lipid membrane. In: A. Leitmannova Liu., ed., Advances in lipid biolayers and liposomes, Planar lipid bilayers (BLM’s) and their applications, Elsevier in press

Lele TP and Ingber DE, Focal adhesions: self-assembling nanoscale mechano-chemical machines that control cell function. In: Nair L, Laurencin C, Halberstadt C, Gonsalves K, eds., Biomedical Nanostructures, John Wiley & Sons ­ In Press.

2006

Lele TP, Pendse J, Kumar S, Salanga M and Ingber DE,2006, Mechanical forces alter zyxin unbinding kinetics within focal adhesions in living cells, Journal of Cellular Physiology, 207:187-94.    PDF

Lele TP, Wagner S, Nickerson J and Ingber DE, 2006, Methods for measuring rates of protein binding to insoluble scaffolds in living cells: Histone H1-chromatin interactions, Journal of Cellular Biochemistry, 99(5):1334-42.    PDF

Lele TP, Thodeti C and Ingber DE, 2006, Force meets chemistry: analysis of mechanochemical conversion in focal adhesions using Fluorescence Recovery After Photobleaching, Journal of Cellular Biochemistry, 97:1175-83.    PDF

Sharp D, Mancini MG, Hinojos C, Dai F, Berno V, Szafran A, Smith KP, Lele TP, Ingber DE and Mancini MA, 2006, Estrogen-receptor-a exchange and chromatin dynamics are ligand- and domain- dependent, Journal of Cell Science, 119:4101-4116.    PDF

Kumar S, Maxwell IZ, Heisterkamp A, Polte TR, Lele TP, Salanga M, Mazur E, Ingber DE, 2006, Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization and extracellular matrix mechanics, Biophysical Journal, 90:3762-73.    PDF

Lele TP and Ingber DE, 2006, A mathematical model to determine molecular kinetic rate constants under non-steady conditions using FRAP, Biophysical Chemistry, 120(1):32-35.    PDF

2005

McMillan N, Lele TP, Snively C and Lauterbach J,2005, Subsurface oxygen on Pt(100): experiments and modeling, Catalysis Today, 105:244-253.

2004

Lele TP, Oh P, Nickerson J and Ingber DE, 2004, An improved mathematical approach for determination of molecular kinetics in living Cells with FRAP, Mechanics and Chemistry of Biosystems, 1:181-190.    PDF

2003

Lele TP, Lauterbach J, and Ramkrishna D, 2003, Mathematical Analysis of physicochemical processes on catalytic surfaces, AICHE J, 49:2158-2172.    PDF

Desai R, Lele TP and Viens F, 2003, A Monte Carlo method for portfolio optimization under partially observed stochastic volatility, IEEE international conference on computational intelligence for financial engineering, 257-263.    PDF

2002

Lele TP and Lauterbach J, 2002, Spatio-temporal pattern formation during CO oxidation on Pt(100) at low and Intermediate Pressures: A Comparative Study, Chaos, 12:164-171.    PDF

2001

Lele TP and Lauterbach J, 2001, Spatio-Temporal Pattern Formation During CO Oxidation on Pt(100) at Elevated Pressures, AICHE J, 47:1418-1424.    PDF